Nay Chi Khin

Nay Chi has a background of molecular biology and protein biochemistry. She has CRISPR editing experience in mice, mammalian cell cultures and model plant species, such as Arabidopsis, liverworts and mosses. Nay Chi’s vision during her time in the Centre is to set up the pipeline of precise gene editing protocols in canola and chickpeas with optimised transformation and regeneration of plants.

Project Description

This project aims to enhance the resilience and productivity of Brassica napus by addressing two critical agricultural challenges: drought tolerance and pod shatter resistance (PSR). Drought tolerance is crucial for sustaining yields under water-limited conditions and involves traits such as water-use efficiency, growth timing, and energy expenditure. Canola plants experience significant reductions in yield and oil content when exposed to water-deficit conditions, particularly during reproductive stages. To address this, a population of canola plants with different levels of water-use efficiency is being studied to understand how some plants conserve water more effectively. This includes examining traits such as the stomatal (leaf pores) characteristics, leaf thickness, and dark respiration (how plants regulate energy use at night). Identifying genetic markers associated with these traits will provide a deeper understanding of the mechanisms that enable canola to use water efficiently and adapt to drought stress. 

PSR is equally vital for minimizing seed loss during harvest and storage. Brassica carinata, a genetically diverse and agriculturally valuable crop, offers a promising resource for improving PSR in B. napus, which has limited genetic variation for this trait. By studying the genetic differences between these species l and analysing natural variation in PSR traits, this project aims to introduce beneficial traits into B. napus. In addition, the project will examine how specific genes influence PSR by identifying differences in gene activity linked to this trait. This includes correlating genetic variations with PSR phenotypes, assessing their impact on gene expression, performing differential gene expression analyses of PSR-related genes, and investigating the functional roles of key regulatory genes in pod shatter resistance. 

By integrating genetic insights into drought tolerance and PSR, this research aims to develop B. napus varieties that are more resilient to environmental stresses and less prone to yield losses. These advancements will enhance yield stability, adaptability, and sustainable agricultural productivity in diverse and challenging environments.